NOVEL HIGH-TITRE RETROVIRAL VECTOR AND TRANSDUCTION METHODS

摘要

The invention relates to a novel high-titre retroviral vector and transduction methods. More specifically, the invention relates to novel retroviral vectors that can produce high-titre, non-replicative, infectious retroviral particles and to methods of using said particles for the transfer of gene constructions of interest. In particular, the invention relates to retroviral vectors which are derived from MoMLV and which comprise a nucleotide sequence that is characterised by general formula: 5'-[CMV-R-U5]-[PBS]-[SD]-Psi+-[MCS2]-[SA]-[MCS]-[PPT]-[LTR]-3', as well as the uses of said vectors.