摘要 |
PROBLEM TO BE SOLVED: To provide a method for synthesizing a protein targeted on or in a membrane structure using a method for synthesizing a cell-free protein, for further detail, the method for synthesizing the protein having a physiological activity comprising adding a cellular membrane component to a cell-free protein reagent without containing an oxidizable type substance under reducing conditions. SOLUTION: This method for synthesizing the protein targeted on or in the membrane structure comprises adding the cellular membrane component to the cell-free protein reagent satisfying the following conditions (a) and (b). (a) without containing a glutathione component at≥2 mM concentration and (b) containing a reducing agent at≥0.2 mM concentration. COPYRIGHT: (C)2005,JPO&NCIPI
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