摘要 |
Macrolides particularly erythromycins and azithromycins, having O-mycaminosy l or O-angolosaminyl groups, particularly at the 5-position, are produced usin g a gene cassette comprising a combination of genes which, in an appropriate strain background, are able to direct the synthesis of mycaminose or angolosamine and to direct its subsequent transfer to an aglycone or pseudoaglycone. Synthetic genes may comprise one or more of angMIII, angMI, angB, angAI, angAII, angorf14, angorf4, tylMIII, tylMI, tylB, tylAI, tylAII, eryCVI, spnO, eryBVI, eryK, tyl Ia and ery G. Glycosyltransfer genes may comprise one or more of eryCIII, tylMII, angMII, desVII, eryBV, spnP and mid I |
申请人 |
PFIZER INC., A CORPORATION ORGANIZED UNDER THE LAWS OF THE STATE OF DELA WAR;BIOTICA TECHNOLOGY LIMITED |
发明人 |
GAISSER, SABINE;MCARTHUR, HAMISH ALASTAIR IRVINE;HAYDOCK, STEPHEN FREDERICK;LEADLAY, PETER FRANCIS |