发明名称 |
Method of determining a bacterium species |
摘要 |
The present invention provides a rapid and precise test for objectively determining a bacterium species. The assay is applicable to sample isolated from blood, serum, other bodily fluids or environmental samples. The detection method comprises the steps of annealing a region in a DNA template to a specific oligonucleotide primer set comprising SEQ-FOR and SEQ-REV in a complimentary fashion, said primer set designed to give rise to a PCR product having a predetermined size dictated by a complimentary primer set; amplifying said region of DNA template by performing a polymerase chain reaction to produce said PCR product; determining a nucleatide sequence of said PCR product; and comparing said PCR product to a DNA sequence in a database to determine a species of bacterium.
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申请公布号 |
US2005130168(A1) |
申请公布日期 |
2005.06.16 |
申请号 |
US20030697802 |
申请日期 |
2003.10.31 |
申请人 |
HAN XIANG-YANG;PHAM AUDREY S. |
发明人 |
HAN XIANG-YANG;PHAM AUDREY S. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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