奈瑟球菌蛋白质的异源表达

摘要

异源表达脑膜炎奈瑟球菌和淋病奈瑟球菌蛋白质的其它和改进的方法。这些方法通常影响表达的水平、纯化的简易性、细胞的定位和/或所表达的蛋白质的免疫特性。

主权项

1.一种异源表达蛋白质的方法，其特征在于，所述的蛋白质中的聚-甘氨酸段发生缺失，所述蛋白质是287、741、983或Tbp2，蛋白质287的序列如下所示： 1 CGGGGGGSPD VKSADTLSKP AAPVVSEKET EAKEDAPQAG SQGQGAPSAQ 51 GSQDMAAVSE ENTGNGGAVT ADNPKNEDEV AQNDMPQNAA GTDSSTPNHT101 PDPNMLAGNM ENQATDAGES SQPANQPDMA NAADGMQGDD PSAGGQNAGN151 TAAQGANQAG NNQAAGSSDP IPASNPAPAN GGSNFGRVDL ANGVLIDGPS201 QNITLTHCKG DSCSGNNFLD EEVQLKSEFE KLSDADKISN YKKDGKNDKF251 VGLVADSVQM KGINQYIIFY KPKPTSFARF RRSARSRRSL PAEMPLIPVN301 QADTLIVDGE AVSLTGHSGN IFAPEGNYRY LTYGAEKLPG GSYALRVQGE351 PAKGEMLAGA AVYNGEVLHF HTENGRPYPT RGRFAAKVDF GSKSVDGIID401 SGDDLHMGTQ KFKAAIDGNG FKGTWTENGS GDVSGKFYGP AGEEVAGKYS451 YRPTDAEKGG FGVFAGKKEQ D蛋白质741的序列如下所示： 1 CSSGGGGVAA DIGAGLADAL TAPLDHKDKG LQSLTLDQSV RKNEKLKLAA 51 QGAEKTYGNG DSLNTGKLKN DKVSRFDFIR QIEVDGQLIT LESGEFQVYK101 QSHSALTAFQ TEQIQDSEHS GKMVAKRQFR IGDIAGEHTS FDKLPEGGRA151 TYRGTAFGSD DAGGKLTYTI DFAAKQGNGK IEHLKSPELN VDLAAADIKP201 DGKRHAVISG SVLYNQAEKG SYSLGIFGGK AQEVAGSAEV KTVNGIRHIG251 LAAKQ蛋白质983的序列如下所示： 1 CLGGGGGGTS APDFNAGGTG IGSNSRATTA KSAAVSYAGI KNEMCKDRSM 51 LCAGRDDVAV TDRDAKINAP PPNLHTGDFP NPNDAYKNLI NLKPAIEAGY101 TGRGVEVGIV DTGESVGSIS FPELYGRKEH GYNENYKNYT AYMRKEAPED151 GGGKDIEASF DDEAVIETEA KPTDIRHVKE IGHIDLVSHI IGGRSVDGRP201 AGGIAPDATL HIMNTNDETK NEMMVAAIRN AWVKLGERGV RIVNNSFGTT251 SRAGTADLFQ IANSEEQYRQ ALLDYSGGDK TDEGIRLMQQ SDYGNLSYHI301 RNKNMLFIFS TGNDAQAQPN TYALLPFYEK DAQKGIITVA GVDRSGEKFK351 REMYGEPGTE PLEYGSNHCG ITAMWCLSAP YEASVRFTRT NPIQIAGTSF401 SAPIVTGTAA LLLQKYPWMS NDNLRTTLLT TAQDIGAVGV DSKFGWGLLD451 AGKAMNGPAS FPFGDFTADT KGTSDIAYSF RNDISGTGGL IKKGGSQLQL501 HGNNTYTGKT IIEGGSLVLY GNNKSDMRVE TKGALIYNGA ASGGSLNSDG551 IVYLADTDQS GANETVHIKG SLQLDGKGTL YTRLGKLLKV DGTAIIGGKL601 YMSARGKGAG YLNSTGRRVP FLSAAKIGQD YSFFTNIETD GGLLASLDSV651 EKTAGSEGDT LSYYVRRGNA ARTASAAAHS APAGLKHAVE QGGSNLENLM701 VELDASESSA TPETVETAAA DRTDMPGIRP YGATFRAAAA VQHANAADGV751 RIFNSLAATV YADSTAAHAD MQGRRLKAVS DGLDHNGTGL RVIAQTQQDG801 GTWEQGGVEG KMRGSTQTVG IAAKTGENTT AAATLGMGRS TWSENSANAK851 TDSISLFAGI RHDAGDIGYL KGLFSYGRYK NSISRSTGAD EHAEGSVNGT901 LMQLGALGGV NVPFAATGDL TVEGGLRYDL LKQDAFAEKG SALGWSGNSL951 TEGTLVGLAG LKLSQPLSDK AVLFATAGVE RDLNGRDYTV TGGFTGATAA1001 TGKTGARNMP HTRLVAGLGA DVEFGNGWNG LARYSYAGSK QYGNHSGRVG1051 VGYRF蛋白质Tbp2的序列如下所示： 1 CLGGGGSFDL DSVDTEAPRP APKYQDVFSE KPQAQKDQGG YGFAMRLKRR 51 NWYPQAKEDE VKLDESDWEA TGLPDEPKEL PKRQKSVIEK VETDSDNNIY101 SSPYLKPSNH QNGNTGNGIN QPKNQAKDYE NFKYVYSGWF YKHAKREFNL151 KVEPKSAKNG DDGYIFYHGK EPSRQLPASG KITYKGVWHF ATDTKKGQKF201 REIIQPSKSQ GDRYSGFSGD DGEEYSNKNK STLTDGQEGY GFTSNLEVDF251 HNKKLTGKLI RNNANTDNNQ ATTTQYYSLE AQVTGNRFNG KATATDKPQQ301 NSETKEHPFV SDSSSLSGGF FGPQGEELGF RFLSDDQKVA VVGSAKTKDK351 PANGNTAAAS GGTDAAASNG AAGTSSENGK LTTVLDAVEL KLGDKEVQKL401 DNFSNAAQLV VDGIMIPLLP EASESGNNQA NQGTNGGTAF TRKFDHTPES451 DKKDAQAGTQ TNGAQTASNT AGDTNGKTKT YEVEVCCSNL NYLKYGMLTR501 KNSKSAMQAG ESSSQADAKT EQVEQSMFLQ GERTDEKEIP SEQNIVYRGS551 WYGYIANDKS TSWSGNASNA TSGNRAEFTV NFADKKITGT LTADNRQEAT601 FTIDGNIKDN GFEGTAKTAE SGFDLDQSNT TRTPKAYITD AKVQGGFYGP651 KAEELGGWFA YPGDKQTKNA TNASGNSSAT VVFGAKRQQP VR。