METHOD AND SYSTEM FOR MOLECULAR INTERACTION ANALYSIS

摘要

The invention relates to a computer-implemented method for determining at least one kinetic parameter for the interaction of an analyte in solution with an immobilized ligand from a data set comprising a plurality of different binding curves, each of which represents the progress of the interaction of the analyte with the ligand with tune, comprising the steps of: a) performing at least one fit of the whole data set or subsets thereof to a predetermined kinetic model for the interaction; b) based on the result of the fit or fats performed in step a), identifying and excluding binding curves of unacceptable quality; c) performing a final fit to the remaining data set; and d) obtaining therefrom the kinetic parameter or parameters. The invention also relates to an analytical system for carrying out the method, as well as a computer program, computer program product and computer system for performing the method.