| 摘要 |
<p>A detection reagent for a thermostable direct hemolysin-related hemolysin gene of Vibrio parahaemolyticus is provided, which detection reagent has improved sensitivity and speed of detection, and is capable of detecting both TRH1 and TRH2 of Vibrio parahaemolyticus. The detection reagent for a thermostable direct hemolysin-related hemolysin(TRH) gene of Vibrio parahaemolyticus comprises an oligonucleotide consisting of at least 10 contiguous bases of the nucleotide sequence set forth in SEQ. ID NO:2, or an oligonucleotide in which one or more of nucleotides in the oligonucleotide consisting of at least 10 contiguous bases of the nucleotide sequence of SEQ. ID NO:2 are deleted, substituted or added and is capable of specifically binding to the specific sequence, as a first primer; and an oligonucleotide consisting of at least 10 contiguous bases of the nucleotide sequence set forth in SEQ. ID NO:1, or an oligonucleotide in which one or more of nucleotides in the oligonucleotide consisting of at least 10 contiguous bases of the nucleotide sequence of SEQ. ID NO:1 are deleted, substituted or added and is capable of specifically binding to the sequence complementary to the specific sequence, as the second primer, wherein the first primer is an oligonucleotide having the nucleotide sequence of SEQ ID NO:2, and the second primer is an oligonucleotide having the nucleotide sequence of SEQ ID NO:1.</p> |
