STANDARDS IN PCR

摘要

The invention provides the use of a standard in a method of nucleic acid sequence-based quantification on a mixture comprising a plurality of constituents, each constituent comprising a common nucleotide sequence, and a constituent of interest having a specific nucleotide sequence, characterised in that the standard is prepared from single said constituent comprising the said common sequence and a constituent-specific sequence in a known or elucidatable fixed copy number ratio.