发明名称 |
Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates |
摘要 |
Improved methods and reagents for chromosome walking of nucleic acid are discussed herein. A library of amplifiable nick translation molecules is generated, and a chromosome walk is initiated from a known sequence in the nucleic acid by producing at least one nick translate molecule, sequencing part of the nick translate molecule, and producing a second nick translate molecule by initiating the primer extension from the region of the obtained sequence of the prior nick translate molecule.
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申请公布号 |
US2005032104(A1) |
申请公布日期 |
2005.02.10 |
申请号 |
US20040909516 |
申请日期 |
2004.08.02 |
申请人 |
MAKAROV VLADIMIR L.;KAMBEROV EMMANUEL;SLEPTSOVA IRINA |
发明人 |
MAKAROV VLADIMIR L.;KAMBEROV EMMANUEL;SLEPTSOVA IRINA |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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