| 摘要 |
PURPOSE: A fusion protein comprising an angiopoietin receptor-binding domain and a multimerization domain is provided, which fusion protein comprises a coiled coil domain capable of forming a dimer or multimer, fused to another domain. CONSTITUTION: A coiled coil type chimeric molecule comprises a coiled-coil domain linked to either a receptor-binding domain of a ligand or a ligand-binding domain of a receptor which forms a biologically active multimer, wherein the non-multimeric form of the chimeric molecule is not biologically active; the coiled coil domain comprises a domain belonging to a protein belonging to matrix protein family, transcription factor family, growth factor family or secretory protein family; the chimeric molecule comprises the coiled coil domain from cartilage matrix protein or cartilage oligomeric matrix protein; the receptor binding domain binds to Tie2 or Tiel receptor; the receptor binding domain is a fibrinogen-like domain of angiopoietin-1, angiopoietin-2, angiopoietin-3 or angiopoietin-4; the coiled coil domain is linked to an extracellular domain of the receptor; the ligand binding domain binds to angiopoietin-1, angiopoietin-2, angiopoietin-3 or angiopoietin-4; and the ligand binding domain comprises extracellular domain of Tie2 receptor or Tiel receptor.
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