| 摘要 |
A method is described for the detection of cytosine methylation in DNA samples. A genomic DNA sample is chemically treated, preferably with a bisulfite (=disulfite, hydrogen sulfite), in such a way that cytosine is converted to uracil, while 5-methylcytosine remains unchanged. Segments of the sample DNA are amplified with at least 2 primers in a polymerase reaction, preferably a polymerase chain reaction. Finally, the fragments are investigated with respect to the base composition of each of the two complementary strands of the amplificate, whereby a conclusion is made on the methylation state in the amplified segment of the genomic DNA sample from the difference in molecular weight of the two strands.
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