Method of forming complex

摘要

A method of forming a complex consisting of a double-stranded nucleic acid and oligonucleotide(s) characterized by comprising: the step of mixing a double-stranded nucleic acid with at least one oligonucleotide to give a reaction mixture, wherein the oligonucleotide is a chimeric oligonucleotide containing at least a member selected from among deoxyribonucleotides and nucleotide analogs and a ribonucleotide and having a sequence substantially complementary to the base sequence of one of the strands of the double-stranded nucleic acid as described above; and the step of incubating the reaction mixture to form a complex under such conditions that the above-described double-stranded nucleic acid is not denatured.