BACTERIAL ADPGLUCOSE PYROPHOSPHATASE, METHOD FOR OBTAINING THE LATTER AND ITS USE IN THE PRODUCTION OF ASSAY DEVICES AND IN THE OBTENTION OF TRANSGENIC PLANTS AND BACTERIA.

摘要

AGPPase is an enzyme catalyzing the hydrolysis of ADPG (adenosine diphosphate glucose). The enzyme obtained from microbial extracts is used in assay devices to determine ADPG levels, based on G1P (glucose-1-phosphate) released by the reaction catalyzed by the AGPPase. The invention also relates to partial sequences of amino acids of the enzyme, the sequence of the gene coding for said enzyme and the deduced protein. The invention further relates to the obtention of transgenic plants and bacteria that overexpress the gene of the AGPPase and which have a high sugar content, a low starch or glucogen content, respectively, and high resistance to high concentration of salts and temperatures.