SUBSTRAT-GEBUNDENE OLIGONUKLEOTIDE.

摘要

PCT No. PCT/GB89/01114 Sec. 371 Date Mar. 20, 1991 Sec. 102(e) Date Mar. 20, 1991 PCT Filed Sep. 21, 1989 PCT Pub. No. WO90/03382 PCT Pub. Date Apr. 5, 1990.A method for synthesizing oligonucleotides by solid-phase methodology wherein the linkage between the oligonucleotide and the solid phase support is a non-labile phosphodiester. The spacer arm between the support and the phosphodiester is a hydrophilic group. The product of this method is an immobilized oligonucleotide which can be used in solid phase hybridization assays.