COMPOSITIONS AND METHODS FOR HYDROXYLATING EPOTHILONES
摘要
Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.
申请公布号
WO2004078978(A1)
申请公布日期
2004.09.16
申请号
WO2003US06108
申请日期
2003.02.26
申请人
BRISTOL-MYERS SQUIBB COMPANY;BASCH, JONATHAN, DAVID;CHIANG, SHU-JEN;LIU, SUO-WIN;NAYEEM, AKBAR;SUN, YUHUA;YOU, LI
发明人
BASCH, JONATHAN, DAVID;CHIANG, SHU-JEN;LIU, SUO-WIN;NAYEEM, AKBAR;SUN, YUHUA;YOU, LI