IDENTIFICATION OF ANTIGEN EPITOPES

摘要

The invention relates to methods for the identification and/or for detection of T-cell-epitopes of a protein antigen, methods for the production of peptide vaccines against a protein antigen, methods for the quality control of receptor ligand complexes and/or components thereof, methods for the production of nanoparticles comprising at least one immobilised receptor unit or an immobilised receptor, methods for the production of nanoparticles comprising immobilised receptor ligand complexes, especially MHC molecules comprising a peptide, methods for the enrichment of and/or isolation of specific CD4<+>-T- or CD8<+>-T-lymphocytes from peripheral mononuclear blood cells, methods for the priming a CD8<+>-T-lymphocyte reaction in vitro, nanoparticle having an immobilised receptor unit, especially an immobilised chain of an MHC-molecule, nanoparticles having an immobilised receptor, especially an immobilised MHC-molecule, nanoparticles having an immobilised receptor ligand complex, especially an MHC molecule comprising a peptide, a peptide vaccine, a kit for the identification and/or detection of T-cell epitopes of a protein antigen, and the use of nanoparticles in the identification and/or detection of T-cell epitopes, for the production of peptide vaccines in order to enrich and/or isolate specific T-lymphocytes and for priming a CD8<+>-T-lymphocyte reaction in vitro.