FUNGAL BIOSENSOR AND ASSAY

摘要

A fungal biosensor comprising luminescent or fluorescent filamentous fungi is provided. The luminescence/fluorescence of the fungi varies depending upon whether the predetermined test substance is present or absent. Typically the gene encoding the luminescent or fluorescent protein is genetically engineered to be under the control of an inducible promoter or enhancer sensitive to the test substance. Suitable fungi include Neurospora Sp., and Aspergillus Sp. Exemplary luminescent proteins include the Gaussia luciferase and the phosphoprotein obelin. An assay using the biosensor is also described.