| 摘要 |
The present invention concerns a method for the detection of cytosine methylation in DNA samples, in which the following steps are conducted: a genomic DNA sample which comprises target DNA and background DNA is chemical ly treated such that all unmethylated cytosine bases are converted to uracil, while the 5-methylcytosine bases remain unchanged; the chemically treated DN A sample is amplified with the use of at least 2 primer oligonucleotides as we ll as a polymerase and a nucleotide mixture, the composition of which leads to a preference for the target DNA over the background DNA as the template; and t he methylation state in the target DNA is concluded from the presence of an amplificate or its quantity.
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