MONOCLONAL ANTIBODY RECOGNIZING BOTH HUMAN ERYTHROPOIETIN(EPO) AND MODIFIED ERYTHROPOIETIN AND HYBRIDOMA CELL LINE PRODUCING THE SAME

摘要

PURPOSE: A monoclonal antibody recognizing both human erythropoietin(EPO) and modified erythropoietin and a hybridoma cell line producing the same are provided, thereby effectively purifying EPO and modified EPO using chromatography. CONSTITUTION: The hybridoma cell KCLRF-BP-00071 produces the monoclonal antibody recognizing both human erythropoietin(EPO) and modified erythropoietin. The method for preparing the hybridoma cell KCLRF-BP-00071 producing the monoclonal antibody recognizing both human erythropoietin(EPO) and modified erythropoietin comprises the steps of: immunizing a mouse with human EPO to obtain human EPO antibody, fusing the human EPO antibody with NS-O, and culturing the fused cells on wells to obtain hybridoma cells producing monoclonal human EPO antibody; adding first clones into the hybridoma cells and culturing them to obtain hybridoma cells producing monoclonal human EPO antibody; and selecting hybridoma cells producing monoclonal human EPO antibody recognizing both human erythropoietin(EPO) and modified erythropoietin.