发明名称 |
TYROSYL-TRNA SYNTHASE VARIANTS |
摘要 |
Tyrosyl-tRNA synthetases having a modified amino acid sequence whereby unnatural amino acids can be more efficiently incorporated than original natural amino acids. More specifically, tyrosyl-tRNA synthetases having been modified at the amino acid(s) at the 37- and/or 195- positions. A method of modifying tyrosyl-tRNA synthetase characterized in that the position of an amino acid to be modified is determined based on the 3-D structure of a tyrosyl-AMP and of tyrosyl synthetase complex and an amino acid to which this enzyme binds.
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申请公布号 |
EP1424395(A1) |
申请公布日期 |
2004.06.02 |
申请号 |
EP20020794635 |
申请日期 |
2002.01.11 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
KIGA, DAISUKE;SAKAMOTO, KENSAKU;HIRAO, ICHIRO;YOKOYAMA, SHIGEYUKI |
分类号 |
C12N15/52;C07B61/00;C12N1/21;C12N9/00;G01N33/48;G06F17/30;G06F17/50;(IPC1-7):C12N15/52 |
主分类号 |
C12N15/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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