| 摘要 |
<p>Discriminating dysplastic cells overexpressing INK4a gene products (G) from other cells expressing (G) by determining co-expression of (G) (marker M1) and cell proliferation marker (marker M2) in a single cell, where over-expression of M1 and expression of M2 at detectable level indicates dysplastic state, and over-expression of M1 and expression of marker for senescence or apoptosis at detectable level is indicative of non-dysplastic state. Discriminating dysplastic cells overexpressing INK4a gene products (G) from other cells expressing (G) at a detectable level in biological samples involves determining in a cytological or histological testing procedure the co-expression of at least two marker molecules in at least one single cell, where at least one marker molecule is an expression product encoded by the INK4a gene and at least one further marker molecule is a cell proliferation marker, where the over-expression of at least one (G) and expression of at least one marker for active cell proliferation at a detectable level within the single cell is indicative of the dysplastic state of the cell, and where the over-expression of at least one (G) and expression of at least one marker for senescence, terminal cell differentiation, apoptosis or cell cycle arrest at a detectable level within the single cell is indicative of the non-dysplastic state of the cell. An independent claim is also included for a kit for performing the above method, which is a diagnostic kit or a research kit, comprising at least one or more probes for the detection of the presence or absence and/or the level of the over-expression of at least one INK4a gene-product and at least one cell proliferation marker gene product in biological samples.</p> |
