| 摘要 |
<p>Identifying microbes causing infection in clinical specimens in which phagocytes are isolated from the clinical specimen and immobilized. The phagocytes are then treated to enhance their membrane permeability with lysostaphin, lysozyme, N-acetylmuramidase, or zymolase. The exposed DNA is then hybridized with a DNA probe. Identifying microbes causing infection in clinical specimens in which phagocytes are isolated from the clinical specimen and immobilized. The phagocytes (5 x 10 6> to 1 x 10 8> cells/ml) are then treated to enhance their membrane permeability, by treatment with lysostaphin (1-1000 U/ml), lysozyme (10 3> to 10 6> U/ml), N-acetylmuramidase (10 to 10 4> U/ml) or zymolase (50-500 U/ml). The treated phagocytes, in which DNA derived from the infective microorganism and contained within the phagocyte is exposed, is then hybridized with a DNA probe (0.1 to 22 ng/mu l) (350-600 bases in length) hybridizing stringently to the microbial DNA, in the presence of a surfactant. An independent claim is included for kits for carrying out the method, containing the treatment enzyme and the DNA probe.</p> |
