Real-time PCR assay of the bacterium Edwardsiella ictaluri in channel catfish

摘要

A rapid and sensitive PCR-based assay is provided to facilitate early detection of Edwardsiella ictaluri in channel catfish. This bacteria is the causative agent in the disease, Enteric septicemia of catfish (ESC). Also provided is a method of selecting breeding stock for use in selective breeding programs to improve disease resistance in channel catfish. Also provided is a method of determining the efficacy of vaccines produced against ESC.