摘要 |
PROBLEM TO BE SOLVED: To provide antibodies against ginkgolic acid which is a ginkgo-derived contact allergy-inducing component, and its related compounds and an analytic method by using them. SOLUTION: The antibodies against ginkgolic acid and its related compounds are obtained by cleaving a part of the ginkgolic acid to obtain an aldehyde compound, bonding the carboxy group of the aldehyde compound with the lysine residue of a protein such as bovine serum albumin (BSA) by carbodiimide method using EDC to impart antigenic property, immunizing an animal with the obtained material, isolating spleen after the immunization, fusing with myeloma cells by using a polyethylene glycol, selecting only fused cells (hybridomas) by using an HAT medium, cloning the hydridomas by a limiting dilution method and isolating hybridomas selected by using the aldehyde compound-human serum albumin (HSA) complex and producing anti-ginkgolic acid monoclonal antibody (MAb). The quantitative analysis method is performed by a labeled immuno-quantitative determination method. COPYRIGHT: (C)2004,JPO
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