| 摘要 |
PURPOSE: A regenerating method of immobilized enzyme is provided, thereby effectively using the residual activity of an immobilized enzyme spent for lipolysis, and regenerating the spent immobilized enzyme to have a performance similar to that before the lipolysis at a low cost using less amount of liquid waste. CONSTITUTION: The method for regenerating an immobilized enzyme for lipolysis which exhibits a reduced activity after having been used for lipolysis comprises the steps of: (a) washing the immobilized enzyme comprising fatty acids with a solvent; (b) controlling an equilibrium concentration of the fatty acids in the solvent; (c) removing the washed immobilized enzyme therefrom, and (d) contacting the resulting immobilized enzyme with a fresh enzyme, wherein the fresh enzyme adsorbs onto the immobilized enzyme, wherein the equilibrium concentration of the fatty acids in the washing liquid is adjusted to fall within a range of from about 4 to 28 wt. %; and the solvent is selected from the group consisting of ethanol, n-hexane, and mixtures thereof.
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