GLYCOSYLATED HUMAN GRANULOCYTE COLONY STIMULATING FACTOR(G-CSF) ISOFORM

摘要

PURPOSE: A glycosylated human granulocyte colony stimulating factor(G-CSF) isoform is provided. The glycosylated hG-CSF isoform has improved stability in the human body, therefore it reduces dose and dosage frequency thereof when clinically applied. CONSTITUTION: A glycosylated human granulocyte colony stimulating factor(G-CSF) isoform is prepared by forming one or more sequences of Asn-X-Ser/Thr(N-X-S/T) on the amino acid residues: -T1-P10 (T1-P-L-G-P-A-S-S-L-P10), -Y39-L71 (Y39-K-L-C-H-P-E-E-L-V- L-L-G-H-S-L-G-I-P-W-A- P-L-S-S-C-P-S-Q-A-L-Q-L71); -L92-L99 (L92-E-G-I-S-P-E-L99); and -G125-S142 (G125-M-A-P-A-L-Q-P-T- Q-G-A-M-P-A-F-A-S142), and followed by glycosylation, wherein and glycine at amino acid position 51 is substituted by asparagine, glycine at amino acid position 94 is substituted by asparagine, threonine and glycine at amino acid position 133 and 135 are substituted by asparagine and serine, respectively, or all of them are modified.