METHOD FOR PREPARING OLIGONUCLEOTIDES

摘要

A method for preparing an oligonucleotide comprising the steps ofa) providing a 3 -protected compound having the formula: whereinB is a heterocyclic baseR2 is H, a protected 2 -hydroxyl group, F, a protected amino group, an O-alkyl group, an O-substituted alkyl, a substituted alkylamino or a C4'- O2'methylen linkage R3 is OR'3, NHR''3, NR''3R'''3, a 3'-protected nucleotide or a 3'-protected oligonucleotide,R'3 is a hydroxyl protecting group,R''3, R'''3 are independently an amine protecting group, b) reacting said compound with a nucleotide derivative having a 5 -proctection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bondc) optionally processing the elongated oligonucleotide with a P(III)-internucleotide bond by either or both of steps c1) and c2) in any sequencec1) capping preferably by reacting with a solid supported capping agentc2) oxidizing preferably by reacting the oligonucleotide with a solid supported oxidizing reagent d) removing the 5' -protection group.