Biosensor with compartmentalised reaction and measurement chamber attachment

摘要

Disposable bio-sensor test strip has a bonded structure forming channels, and a reaction/measurement chamber which is positioned over the sensitive surfaces, and taking defined sample volumes. The bio-sensor, as a flat sensor or test strip (1a), has an upper compartment structure (2). It has a sample holding channel (3) with a surfactant on its inner surface or porous hydrophilic filling and an inflow opening, a reaction/measurement chamber (4) where the mean cross section is at least twice as deep and/or wide as the sample channel, and an enzyme or enzyme system which recognizes the analyte, together with an electron mediator. A sample stop channel (5) with hydrophobic surface coating or porous filling material has a mean cross section which is at lest half as deep and/or wide as the outlet opening from the reaction/measurement chamber. The sample collector zone (6) has a large volume capacity with a mean cross section at least twice as deep and/or wide as the stop channel. The channels and chamber are all interconnected. The structure is irreversibly bonded to the sensor or test strip so that the reaction/measurement chamber is placed over the sensitive surfaces (1b,1d) of the sensor or test strip. The sample holding and stop channels have a volume capacity of 0.005-0.05 microliters, the reaction/measurement chamber holds 0.1-1.0 microliters and the sample collector zone has a larger volume of at least 10 microliters.