METHOD CAPABLE OF BEING AUTOMATED FOR DETECTION OF PRP<RES> AND USES THEREOF

摘要

The invention concerns a method capable of being automated for detecting PrP in a biological sample and its uses. Said detection method uses a solid support whereon plasminogen is immobilized. It comprises essentially the following steps: (a) a step which consists in preparing the biological sample during which the latter is incubated on a homogenizing buffer which comprises an ionic or nonionic surfactant, a glucose-containing buffer, a saccharose-based buffer and a PBS buffer or in a capture buffer comprising an ionic surfactant; said step optionally comprises incubation with a proteinase K at a final concentration ranging between 1 and 8 mu g/ml, preferably at a final concentration ranging between 2 and 4 mu g/ml; (b) a step which consists in capturing the PrP on said solid support, which must necessarily be carried out in the presence of a capture buffer, as defined above; (c) a controlled denaturation step of the PrP fixed on said support comprising incubation with a denaturation buffer including at least one chaotropic agent, at a temperature ranging between room temperature and 100 DEG C; (d) a step which consists in detecting the denatured PrP fixed on said support with a PrP protein-specific antibody.