发明名称 |
Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby |
摘要 |
The present invention intends to find out a gene participating in addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus Pichia and provide a means of controlling the same. The present invention also intends to provide a process for producing a protein with reduction of an acidic sugar chain by using the thus controlled yeast strain belonging to the genus Pichia. Namely, the present invention includes a protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein; a gene coding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genus Pichia having been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced.
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申请公布号 |
US2004014170(A1) |
申请公布日期 |
2004.01.22 |
申请号 |
US20020276075 |
申请日期 |
2002.12.09 |
申请人 |
MIURA MASAMI;HIROSE MASAAKI;MIWA TAEKO;IRIE HIROYUKI;KUWAE SHINOBU;MIYANO KENMI;OTANI WATARU;OHI HIDEYUKI |
发明人 |
MIURA MASAMI;HIROSE MASAAKI;MIWA TAEKO;IRIE HIROYUKI;KUWAE SHINOBU;MIYANO KENMI;OTANI WATARU;OHI HIDEYUKI |
分类号 |
C12N1/19;C12N9/12;C12P21/00;(IPC1-7):C12P21/02;C12N1/18;C07H21/04;C07K14/39;C12N15/74 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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