| 摘要 |
It is intended to provide TLR1 knockout mice specifically recognizing a mycobacteria-origin lipoprotein/lipopeptide, which are useful in clarifying the role of TLR1 in vivo, and a method of screening a promoter or an inhibit or for a response to a mycobacteria-origin lipoprotein/lipopeptide with the use of the same. The TLR1 knockout mice are constructed by isolating a TLR1 gene from a mouse gene library, substituting a part of the TLR1 gene containing t he intracellular domain and the transmembrane domain by a neomycin tolerance gene, transferring an HSV-tk gene encoding thymidine kinase into the 3~- terminal side thereof, screening an ES cell clone double-resistant to G418 a nd ganciclovir, injecting the ES cell clone into the blastocyst of a C57BL/6 mouse and then getting the newborns from the reproductive system thereof.</S DOAB>
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