METHOD FOR ISOLATING AN ALLOSTERIC EFFECTOR OF A RECEPTOR

摘要

The invention concerns a method for isolating an allosteric effector of a receptor, by determining the variation of the dissociation kinetics of the complex formed between said receptor and one of its ligands in the presence of said allosteric effector, as compared to the kinetics dissociation formed between said receptor and said ligand, in the absence of said effector, and/or the amplitude of the linkage formed between said receptor and one of its ligands in the presence of said allosteric effector, as compared to the amplitude of the linkage formed between said receptor and said ligand in the absence of said effector, said receptor and said ligand being involved in at least one biological response in suitable physiological conditions, and the allosteric effector being capable of modulating at least one of the responses, said receptor being marked by at least one fluorescent protein, said ligand being marked by a marker consisting either of a molecule capable of absorbing light emitted by the fluorescent protein, or by a fluorescent substance, said steps for determining kinetics dissociation variation and amplitude variation being carried out by fluorescence energy transfer.