发明名称 |
A METHOD OF AND APPARATUS FOR SCREENING TO IDENTIFY DRUG CANDIDATES |
摘要 |
An apparatus is disclosed for screening a compound by monitoring its interactions with a specimen having fluorophore loaded target cells. One embodiment comprises an optical illumination unit comprising a light source wherein light from the light source is directed to illuminate the specimen; a fluorescence discrimination unit which is coupled to receive emitted light from the specimen and separate at least three emitted wavelengths of light from said emitted light; and a fluorescence detection unit which is coupled to the fluorescence discrimination unit counts photons emitted by the wavelengths of emitted light. A second embodiment comprises a two-dimensional acousto-optical scanning system for use in the apparatus for screening drug candidates is also disclosed. The two dimensional acousto-optical scanning system is based on two perpendicular acousto-optical modulators, spaced so that each is within the range of deflection of the first order beams of the other modulator. A method of screening a compound by monitoring its interactions with a specimen having fluorophore loaded target cells is also described. The method comprises the steps of coupling a light source to the specimen to illuminate the specimen; separating at least three wavelengths of light emitted by the specimen; and detecting photon counts from the three emitted wavelengths of light.
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申请公布号 |
WO03104876(A1) |
申请公布日期 |
2003.12.18 |
申请号 |
WO2003US18437 |
申请日期 |
2003.06.11 |
申请人 |
BIOTECHPLEX CORPORATION |
发明人 |
MAO, HUA;WONG, LID, B.;CHANDRA, TARUN;YEATES, DONOVAN, B. |
分类号 |
G01N21/64;G01N33/15;G01N33/483;G01N33/50;G01N33/84;(IPC1-7):G02B26/08;G01J3/30;G01N21/17;G01N33/53;G01N33/533;G01N33/543;G01N35/00 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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