RAPID DETERMINATION OF PROTEIN GLOBAL FOLDS

摘要

This invention provides a method for rapidly obtaining accurate three-dimensional structure of proteins including large or multi-sub unit proteins, using a combination of NMR analysis of backbone only <13>C, <15>N or <13>C and <15>N isotopically labeled proteins which are optionally also 2H isotopically labeled in the Calpha position protons and residual dipolar coupling measurements in more than one partially aligned state and/or orientation data pertaining to overlapping successive peptide pairs.