| 摘要 |
<p>A detecting method with an improved accuracy of detection of an object specimen, finally achieved by simplifying the step of detecting a biochemical reagin by means of a biochip with good reproducibility and enhancing the accuracy of the operation and its effect at the hybridization and label modification steps without requiring the measuring operator to have an excessively high level of skill. A probe nucleic acid is made to have a three-dimensional structure by adopting a loop structure in such a way that the free end not fixed to the surface of a substrate or a portion where a label is modifiable is located on an electrode of a biochip or near the surface of the electrode on the substrate side, or that the essential portion complementarily connected to the biochemical specimen is located on the substrate side. As a result the probe nucleic acid has a property that it can be basically hybridized with only the object specimen. When the probe nucleic acid is hybridized with the sample specimen, the loop structure is destroyed if the object specimen is present, and the label of only the hybridized probe can be modified. The disturbance noise is reduced because the hybridization and the label modification are selectively carried out at the hybridization and label modification steps, greatly improving the accuracy of detection of the object specimen.</p> |
