发明名称 |
Continuous-flow enzyme assay with mass spectrometry detection |
摘要 |
<p>The present invention relates to an on-line detection method comprising the steps of: contacting an effluent of a fractionation step with a controlled amount of an enzyme; allowing the enzyme to interact with analytes suspected to be present in the effluent; addition of a controlled amount of a substrate for said enzyme; allowing a reaction of the enzyme with the substrate providing one or more modified substrate products; and detection of unreacted substrate, or a modified substrate product using a mass spectrometer.</p> |
申请公布号 |
EP1367133(A1) |
申请公布日期 |
2003.12.03 |
申请号 |
EP20020077146 |
申请日期 |
2002.05.31 |
申请人 |
KIADIS B.V. |
发明人 |
IRTH, HUBERTUS;LETZEL, THOMAS |
分类号 |
G01N27/62;C12Q1/00;C12Q1/48;G01N33/68;G01N35/08;(IPC1-7):C12Q1/00 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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