发明名称 |
Interaction trap system for isolating proteins |
摘要 |
Disclosed is a method for determining whether a first proteins capable of physically interacting with a second protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusions protein, the second fusion protein including the second protein covalently bonded t a weak gene activating moiety; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Such a determination facilitated the isolation of the gene encoding the interacting protein. Also disclosed herein is recombinant Cdil polypeptide, nucleic acid encoding the Cdil polypeptide, and uses thereof.Disclosed is a method for determining whether a first proteins capable of physically interacting with a second protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusions protein, the second fusion protein including the second protein covalently bonded t a weak gene activating moiety; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Such a determination facilitated the isolation of the gene encoding the interacting protein. Also disclosed herein is recombinant Cdil polypeptide, nucleic acid encoding the Cdil polypeptide, and uses thereof.
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申请公布号 |
EP1362913(A2) |
申请公布日期 |
2003.11.19 |
申请号 |
EP20030017549 |
申请日期 |
1993.10.20 |
申请人 |
THE GENERAL HOSPITAL CORPORATION |
发明人 |
BRENT, ROGER, DR.;GYURIS, JENO;GOLEMIS, ERICA |
分类号 |
G01N33/53;A61K38/00;C07K14/39;C07K14/47;C07K16/14;C07K16/18;C12N1/19;C12N9/12;C12N9/16;C12N15/09;C12N15/10;C12N15/12;C12P21/02;C12P21/08;C12Q1/02;C12Q1/68;(IPC1-7):C12N15/10 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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