| 摘要 |
An assay method for determining phosphatase targeting toxins, which inhibit protein phosphatases, comprising contacting a solid support having an immobilized ligand thereon with: (i) a sample suspected of being contaminated with toxin and (ii) a non-immobilized ligand, wherein said immobilized ligand is capable of binding to at least one of said toxins, to said nonimmobilized ligand or to complexes of said toxin and said non-immobilized ligand, and said non-immobilized ligand is capable of binding to at least one of said immobilized ligand, to said toxin or to complexes of said toxin and said immobilized ligand whereby the proportion of said immobilized ligand bound by said toxin, said non-immobilized ligand or complexes of said toxin and said non-immobilized ligand is dependent on the toxin content of said sample and wherein said immobilized ligand is capable of generating directly or indirectly detectable signal when uncomplexed, when complexed by said toxin, when complexed by a complex of said toxin and said nonimmobilized ligand or when complexed by said nonimmobilized ligand or said non-immobilized ligand is capable of generating a directly or indirectly detectable signal when uncomplexed or when complexed, separating a bound fraction from a non-bound fraction; and directly or indirectly determining the nonimmobilized ligand bound to the immobilized ligand (the bound fraction) or non- complexed in aqueous solution (the non-bound fraction); wherein the application of (i) and (ii) to the solid support may be performed separately, sequentially or simultaneously and if separately or sequentially, they can be performed in either order.
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