发明名称 |
Production of recombinant proteins using herpes virus promoters and VP16 transactivators |
摘要 |
Stable cell lines are produced to express high levels of a gene product of interest using VP16, a herpes simplex virus transactivator, and a promoter from herpes simplex virus which is a target for VP16. The transactivator and promoter are introduced to a cell line separately using antibiotic resistance genes as selectable markers on separate vectors.
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申请公布号 |
US6635478(B1) |
申请公布日期 |
2003.10.21 |
申请号 |
US19960641294 |
申请日期 |
1996.04.30 |
申请人 |
G. D. SEARLE & CO. |
发明人 |
HIPPENMEYER PAUL JEROME;HIGHKIN MAUREEN KATHERINE |
分类号 |
C12N5/10;C07K14/52;C07K14/61;C07K14/705;C12N9/10;C12N9/72;C12N15/09;C12N15/869;C12P21/02;C12R1/91;G01N33/50;(IPC1-7):C12N5/16;C12N15/79;C12N15/85 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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