发明名称 |
METHODS OF VIRUS PRODUCTION |
摘要 |
A simple yet effective method of increasing production of a thermo-stable virus, such as adenovirus and picornavirus, is presented. The method entails a temperature shift strategy whereby the culture of host cells are shifted to a sub-optimal temperature for a period of time prior to virus infection or cells are grown at a sub-optimal level for the entire cell expansion process including one or more than one passages of cell growth from cryopreserved cells, followed by a shift back to a more optimal temperature at or near the time of virus infection of the respective host cells. Adaptation of such a temperature shift strategy present a simple yet effective method to substantially increase recoverable virus within a respective host cell/virus production scheme without the need to further manipulate other culture and/or media conditions within an established host cell / virus production scheme.
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申请公布号 |
WO03085138(A1) |
申请公布日期 |
2003.10.16 |
申请号 |
WO2003US09269 |
申请日期 |
2003.03.27 |
申请人 |
MERCK & CO., INC.;XIE, LIANGZHI;GOOCHEE, CHARLES, F. |
发明人 |
XIE, LIANGZHI;GOOCHEE, CHARLES, F. |
分类号 |
A61K39/23;C12N7/00;C12N7/02;C12N15/861;C12Q1/70;(IPC1-7):C12Q1/70 |
主分类号 |
A61K39/23 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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