发明名称 |
Removing deacetylase contaminant from cephalosporin C acylase using cationic surfactant |
摘要 |
An enzyme in a mixture containing the enzyme and a contaminant enzyme is purified by selectively aggregating and precipitating the contaminant enzyme with a surfactant. A deacetylase contaminant is separated from Cephalosporin C acylase using a cationic surfactant in an amount of 0.1 to 0.6%. Surfactants include benzylated or methylated cationic surfactants such as alkyl(palm)dimethylbenzyl ammonium chloride, alkyl(palm)trimethyl ammonium chloride and dodecyltrimethyl ammonium chloride. An immobilized enzyme is regenerated using a protease to remove the enzyme from a carrier. The carrier may be a synthetic adsorbent or an ion exchange resin having pores of 100 nm or less in diameter, and the immobilized enzyme is optionally crosslinked. Immobilized cephalosporin C acylase is regenerated using an alkaline or acidic protease.
|
申请公布号 |
US6630338(B2) |
申请公布日期 |
2003.10.07 |
申请号 |
US20000566721 |
申请日期 |
2000.05.09 |
申请人 |
FUJISAWA PHARMACEUTICAL CO., LTD. |
发明人 |
ASAI KATSUSHI |
分类号 |
C12N9/00;C12N9/80;C12N11/02;C12N11/08;(IPC1-7):C12N9/14;C12N11/14;C12P35/06;C12P35/02 |
主分类号 |
C12N9/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|