STRAIN OF MICROORGANISM ESCHERICHIA COLI AS PRODUCER OF L-THREONINE, METHOD FOR PREPARING L-THREONINE-PRODUCING STRAIN OF ESCHERICHIA COLI AND METHOD FOR PREPARING L-THREONINE (VARIANTS)

摘要

FIELD: genetic engineering, microbiology, amino acids. SUBSTANCE: genetic material is introduced from threonine-producing microorganism in chromosome of E. coli strain. Insertion of non-native promoter in indicated chromosome is carried out before the position in chromosome and in functional association with genes encoding biosynthesis of threonine as shown in fig. 5. Prepared strain is cultured in medium and L-threonine is isolated. Such strain as producer of L-threonine is the strain E. coli NRRL B-21593. Invention allows preparing the strain E. coli producing L-threonine with high yield and effectiveness. EFFECT: improved preparing method of L-threonine. 23 cl, 3 tbl, 5 dwg, 3 ex