发明名称 |
Feeder-free culture method for embryonic stem cell |
摘要 |
Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt. |
申请公布号 |
US2003175956(A1) |
申请公布日期 |
2003.09.18 |
申请号 |
US20020330873 |
申请日期 |
2002.12.24 |
申请人 |
BODNAR ANDREA G.;CHIU CHOY-PIK;GOLD JOSEPH D.;INOKUMA MARGARET;MURAI JAMES T.;WEST MICHAEL D. |
发明人 |
BODNAR ANDREA G.;CHIU CHOY-PIK;GOLD JOSEPH D.;INOKUMA MARGARET;MURAI JAMES T.;WEST MICHAEL D. |
分类号 |
C12N15/09;C12N5/02;C12N5/0735;(IPC1-7):C12N5/08 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|