Analytical method and device for precise analysis with a simple sensor

摘要

An analyte and a reactant 3 that reacts with said analyte either directly or indirectly are allowed to react each other on the analytical areas A of a substrate 1 and signals originating from the reaction are detected. In at least a signal detection step, either a signal generation-related portion 4x or a detector is provided in a portion that is opposed to the substrate 1 and high and low areas are formed in either the substrate 1 or the opposed portion or both in such a manner that the distance from each analytical area A of the substrate to the opposed portion is shorter than the distance from each of the non-analytical areas B of the substrate to the opposed portion, whereby signals originating from the reaction in the analytical areas A will be detected at higher intensities than signals originating from the reaction in the non-analytical areas B. The analyte can be quantitatively or qualitatively analyzed on the analytical areas of the substrate in high precision with reduced effects from the non-analytical areas of the substrate. Simultaneous analysis of multiple samples or simultaneous analysis for multiple items can also be accomplished with a simple sensor configuration. <IMAGE>