| 摘要 |
<p>This invention is directed to a continuous method for measuring the activity of an enzyme that catalyzes the addition of an acetyl group to a residue capable of being acetylated or an enzyme that catalyzes the removal of an acetyl group from an acetylated residue e.g. histone acetyltransferases and histone deacetylase enzymes. Said enzyme is incubated with 1) a protease, 2) a polypeptide comprising a residue in which the acetylation state of the residue modifies the rate of cleavage of the polypeptide by the protease and a moiety that possesses an optical signal that changes upon cleavage of the polypeptide.</p> |
