发明名称 |
Method for the production of cotton somatic embryos |
摘要 |
The present invention provides Inter alia, a method for the production of cotton somatic embryos comprising (a) isolating a totipotent stomatal cell-containing epidermal explant from leaf material excised from a cotton plant; and (b) culturing said explant in a basal medium which comprises an embryogenic callus-inducing quantity of an auxin and a cytokinin under an embryogenic callus inducing intensity of light until embryogenic callus is formed; and (c) sub-culturing said embryogenic callus onto a somatic embryo differentiation media to produce said somatic embryos. Plants may be regenerated from the somatic embryos and in a particular embodiment of the invention said totipotent stomatal cell is transformed, prior to the inducement of embryogenic callus, with a polynucleotide that provides for a desired agronomic trait.
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申请公布号 |
US2003143744(A1) |
申请公布日期 |
2003.07.31 |
申请号 |
US20030220837 |
申请日期 |
2003.01.13 |
申请人 |
DUNWELL JAMES MARTIN;KEITH DEBORAH JANE;NOBRE JOSE MANSO PRETO |
发明人 |
DUNWELL JAMES MARTIN;KEITH DEBORAH JANE;NOBRE JOSE MANSO PRETO |
分类号 |
A01H4/00;C12N15/82;(IPC1-7):C12N5/04 |
主分类号 |
A01H4/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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