发明名称 |
Method to isolate mutants and to clone the complementing gene |
摘要 |
The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.
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申请公布号 |
US6599745(B1) |
申请公布日期 |
2003.07.29 |
申请号 |
US20000613811 |
申请日期 |
2000.07.11 |
申请人 |
DANISCO INGREDIENTS A/S (DANISCO A/S) |
发明人 |
DE GRAAFF LEENDERT HENDRIK;VAN DEN BROECK HENRIETTA CATHARINA;VISSER JACOB |
分类号 |
C12N15/09;C07K14/38;C07K14/47;C12N1/15;C12N1/19;C12N1/21;C12N9/04;C12N9/24;C12N9/88;C12N15/10;C12N15/31;C12N15/63;C12N15/65;C12N15/80;C12Q1/02;C12Q1/68;(IPC1-7):C12N15/65;C12N15/64 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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