METHOD OF RAPIDLY QUANTIFYING HEPATITIS B VIRUS(HBV) DNA THROUGH REAL-TIME PCR FOR DETECTING THE REPLICATION LEVEL OF HBV DNA

摘要

PURPOSE: A method of rapidly quantifying hepatitis B virus(HBV) DNA through real-time PCR for detecting the replication level of HBV DNA is provided, thereby rapidly and cheaply quantifying HBV DNA used for selection of HBV treating agents. CONSTITUTION: A method of rapidly quantifying hepatitis B virus(HBV) DNA through real-time PCR for detecting the replication level of HBV DNA comprises the steps of: heating a HBV producing microorganism-cultured medium at 95 to 100 deg. C for 10 to 15 minutes; adding primers and a probe specific to HBV into the heated sample and real-time PCR amplifying the sample; and detecting the fluorescence signal at 85 to 86 deg. C during the real-time PCR amplification to quantify the initial HBV DNA, wherein the primers specific to HBV are HBV2005 and HBV2122 having the nucleotide sequences of SEQ ID NOs 5 and 6, respectively; and the probe specific to HBV is HBV core having the nucleotide sequence of SEQ ID NO: 3.