发明名称 |
Process for generating genetically modified pearl millet through agrobacterium and biolistic transformation |
摘要 |
The present invention relates to a process for biolistic transformation and regeneration of Pennisetum glaucum (Pearl millet) comprising: a) initiating embryogenic calli formation from the seeds of P glaucam in an MS media containing 5 mg/L of 2,4 D; b) incubating the said calli in dark for a predetermined period; c) sub-culturing the calli on an MS media containing 3 mg/L of 2.4 D; d) incubating said sub cultured calli under fight for a predetermined period; e) subjecting the embryogenic calli to biolistic bombardment with plasmid DNA containing pre-identified genes using a biolistic apparatus; f) allowing the proliferating calli to grow and differentiate into plantlets; g) analysing the expression of said pre-identified genes in the regenerated plantlets using known techniques.
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申请公布号 |
US2003140382(A1) |
申请公布日期 |
2003.07.24 |
申请号 |
US20020204851 |
申请日期 |
2002.12.02 |
申请人 |
PATELL VILLOO MORAWALA;RAYAPURAM NAGANAND;KRISHNAN RANJINI;CHANDRAN DIVYA |
发明人 |
PATELL VILLOO MORAWALA;RAYAPURAM NAGANAND;KRISHNAN RANJINI;CHANDRAN DIVYA |
分类号 |
A01H1/00;C12N15/09;C12N15/82;(IPC1-7):A01H5/00 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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