Method for non-invasive diagnosis of transplantations and transfusions

摘要

It is known that rejection of transplant tissue is accompanied by tissue damage of the donor graft. Apart from donor specific proteins and genomic DNA, it is known, that by tissue damage mitochondrial DNA (mtDNA) will be released into the circulation of the recipient. Based on this knowledge it is the object of the present invention to suggest a new method or process to measure the quantity of cell free circulatory mtDNA in bodily fluids as an indicator or marker of tissue damage and to use mtDNA polymorphisms to discriminate between donor and recipient tissue damage in a transplant setting. The method according to the invention is a non-invasive diagnostic procedure characterised by the following steps: A. Before transplantation d) Obtaining mtDNA containing material from donor and recipient before transplantation. e) Isolation of mtDNA. f) Analysing of the hypervariable regions of the mtDNA for informative polymorphisms which can discriminate between donor and recipient. B. After Transplantation c) Obtaining cell free bodily fluids from a patient. d) Mitochondrial DNA extraction using a commercial procedure. Detection and quantification of donor specific mtDNA in the probe of patient using the donor specific polymorphisms.